Investigation of some concentrations of growth regulators (BAP, GA3, NAA and IBA) in micropropagation of myrobalan 29c (Prunus cerasifera Ehrh.)

Abstract Stone fruits are valuable because of high nutritional. propagation of the same rootstocks is vital in horticulture. The aim of the present study was to investigate some concentrations of growth regulators such as benzylaminopurine (BAP), gibberellic acid (GA3), naphthalene acetic acid (NAA), indole butyric acid (IBA) in shoot germination and rooting of myrobalan 29c. This experiment was performed in a completely randomized block design with three replications (at least 5 samples per replication) in 14 experimental groups (G1-G14). In this study, MS culture medium and growth regulators of BAP (0, 0.5, 1 and 2 mg/L), GA3 (0, 0.1 and 0.2 mg/L), NAA (0 and 0.1 mg/L) was used in shoot generation. The results show that shoot generation varies between 1.05 in G1 group to 8.27 in G6 group. The shoot length varies between 2.87 cm in G5 group to 4 cm in G6 group. The best shoot generation was observed at concentrations of 1 mg/L BAP, 0.1 mg/L NAA and 0.2 GA3 at 8.27 numbers. The 1/2 MS medium and growth regulators of IBA (0, 0.25, 0.5 and 1 mg/L) and NAA (0, 0.25, 0.5 and 1 mg/L) were used for rooting. The results show that rooting varies between zero numbers in the G1 group to 7.6 numbers with 8.78 cm length in the G13 group. The best rooting was obtained at 1/2MS with 0.5 IBA and 0.25 NAA concentration with 7.6 root numbers.